The 6D4 mAb recognizes rhesus ROR1, and ROR1 expression was similar in human and macaque tissues, suggesting that the macaque is a suitable model to evaluate safety of ROR1-targeted therapies. Contrary to previous findings, we found ROR1 is expressed on several normal tissues, including parathyroid pancreatic islets and regions of the esophagus, stomach, and duodenum. The data show that ROR1 is homogenously expressed on a subset of ovarian cancer, triple-negative breast cancer, and lung adenocarcinomas. Results: The 6D4 mAb is a sensitive and specific reagent to detect cell surface ROR1 by IHC. We developed a ROR1-specific monoclonal antibody (mAb) targeting the carboxy-terminus of ROR1 and evaluated its specificity and sensitivity in IHC. Experimental Design: Our studies show that published antibodies lack sensitivity to detect endogenous levels of cell surface ROR1 by immunohistochemistry (IHC) in formalin-fixed, paraffin-embedded tissues. On-target, off-tumor toxicity is a challenge for most nonmutated tumor antigens however, prior studies suggest that ROR1 is absent on most normal tissues. ROR1 is considered a promising target for cancer therapy due to putative tumor-specific expression, and multiple groups are developing antibodies and/or chimeric antigen receptor-modified T cells to target ROR1. They do this by adding Crowd Control effects (like stuns and slows) or bonus damage to the player's character or their attacks.Purpose: This study examines cell surface ROR1 expression in human tumors and normal tissues. These are the items that ultimately increase the players damage output in some way. There are three main categories that these items fall into: Offensive, Defensive and Other. Do note, however, that unless otherwise stated, collecting multiples of the same item with an effect that has a chance of activating will not increase the chances of the effect being triggered (see Ifrit's Horn in the tables below for an example). They can be stacked and do not appear to have a stacking limit, however some seem to have reduced effects when stacked further testing is required to determine how much each item is affected. The majority of the items in this game are equipped passively and are always active. Items take 30 seconds to disappear when left on the ground. Boss enemies will always drop an item when killed, and each Boss has a chance of dropping an item that is unique to them. Shrines (both the gold and health variants) can also give the player items, however this is not guaranteed. Large Containers also have a chance (albeit small) to have an item inside them along with the standard health, gold and experience. Items can be found inside Chests, Shops and Roulette Chambers. Each item has a colored border around them that indicates their rarity:Ģ - although usable items technically have their own rarity as dictated by their border, they actually have individual drop rates (for example, a Captain's Brooch has a lower likelihood of dropping than a Foreign Fruit) There is no limit to how many items the player can hold at once.

A complete Item Log For the items in Risk of Rain 2, see Item (Risk of Rain 2).Īn Item is a randomized collectible that spawns over the course of the game.

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